规格：     0.1ml

Anti-PI3K P110 alpha,磷脂酰肌醇激酶PI3K P110 alpha抗体 7折促销
Specificity / Sensitivity

PI3 Kinase p110α (C73F8) Rabbit mAb detects endogenous levels of total PI3K p110α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Asp520 of the sequence of human PI3K p110α.

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
4. Stoyanov, B. et al. (1995) Science 269, 690-3.

Application References

• Popkie, A.P. et al. (2010) J Biol Chem 285, 41337-47. Applications: Western Blotting
• Yoshioka, K. et al. (2012) Nat Med 18, 1560-9. Applications: Western Blotting
• Biswas, K. et al. (2013) J Biol Chem 288, 2325-39. Applications: Western Blotting