FITC Annexin V Apoptosis Detection Kit with 7-AAD 640922  100T

Reactivity:     All mammalian species
Storage & Handling:     Store between 2°C and 8°C. Do not freeze.

Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Recommended Usage:     

Staining Procedure:
1. Wash cells twice with cold BioLegend's Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 5 µl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.