Anti-Human CD11b (activation epitope) FITC

11-0113-41
25tests

USA

The CBRM1/5 monoclonal antibody reacts with an activation-specificepitope of

human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophilsand monocytes
after stimulation with chemoattractants or phorbol esters but does notrecognize Mac-1
on resting myeloid cells. Through interactions with its ligands, Mac-1participates in
adhesive cell interactions. The epitope recognized by this mAblocalizes to the I domain
on the α chain of Mac-1 very close to the ligand binding site in aregion that is
widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogenand ICAM-1 and
inhibits chemoattractant-stimulated adhesion of eosinophils to theIntercellular Adhesion
Molecule-1 (ICAM-1). It should be noted that low level activation mayoccur during processing
of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit somebinding to Mac-1
in these unstimulated samples. However, higher levels of Mac-1expression are observed in
activated samples when compared to unstimulated cells.