β-半乳糖苷酶染色试剂盒  9860S  1 Kit (125 35mm wells)

Product Includes     Quantity (with Count)                 
10X Fixative Solution     15 ml                 
10X Staining Solution     15 ml                 
100X Solution A     1.5 ml                 
100X Solution B     1.5 ml                 
X-Gal     150 mg

The Senescence β-Galactosidase Staining Kit is designed to detect β-galactosidase activity at pH 6, a known characteristic of senescent cells not found in presenescent, quiescent or immortal cells (3). The kit includes all reagents necessary for this assay.

Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent beta-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.

1.  Goldstein, S. (1990) Science 249, 1129-1133.

2.  Sherwood, S. W. et al. (1988) Proc. Natl. Acad. Sci. , USA 85, 9086-9090.

3.  Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-9367.

4.  Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.

5.  Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-3251.

Product Specific References

Su, D. et al. (2009) J Biol Chem 284, 12153-64.

Swarbrick, A. et al. (2008) Proc Natl Acad Sci U S A 105, 5402-7.

Efeyan, A. et al. (2007) Cancer Res 67, 7350-7.

Baker, D.J. et al. (2004) Nat Genet 36, 744-9.

Boucher, M.J. et al. (2004) Am J Physiol Gastrointest Liver Physiol 286, G736-46.

Efeyan, A. et al. (2009) PLoS One 4, e5475.

Chang, C.J. et al. (2011) Nat Cell Biol 13, 317-23.

Delmore, J.E. et al. (2011) Cell 146, 904-17.

Blasco, R.B. et al. (2011) Cancer Cell 19, 652-63.