300908 PE anti-human CD8a 100 tests

Isotype:     Mouse IgG1, κ
Isotype Control:    PE Mouse IgG1, κ Isotype Ctrl
Workshop
Number:     V CD08.10
Reactivity:     Human, Cross-Reactivity: Chimpanzee, Marmoset
Formulation:     Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation:     The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:     The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:     

FC - Quality tested
Application
Notes:     

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6.
Application
References:     

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)